162 The Effect of Innate Immune Receptor Activation on Differential Breed Responses to<i>Haemonchus Contortus</i> in Sheep

Abstract Previous studies have indicated the importance of Nod-like receptor pyrin containing domain 3 (NLRP3) to early immune response in St. Croix (STC) sheep. NLRP3 is important Th2 response, which classical Haemonchus contortus larval antigen (HcLA). driven by interleukin (IL) -4 and IL-13 cytokine production prevents parasite establishment further resistance as seen STC LPS, activation. Distinct differences been structure between both SUF. upregulates inflammatory genes NLRP3, TLR4 TLR2 while SUF showed delayed responses leading their susceptibility. The objective this study was investigate role differential breed context Hc infection. P Peripheral blood mononuclear cells (PBMC) from were isolated stimulated with HcLA only, combination pharmacological inhibitor (MCC950) complete media for 6 hours. Gene expression revealed that treatment MCC950 upregulated (P &amp;lt; 0.001), STAT6 IL-4 IL-10 0.001) PBMC an increase IL-1β 0.001). This indicates inhibition improves confirms STC.